Roanoke Island served as the first English colony in the New World. There were two attempts to colonize the island, but both failed. The last one – and the most mysterious – happened in the late 1580s. The colony was in need of supplies, so John White took his ship back to England to replenish their stock. Having returned much later than he expected though – 3 years later, instead of 6 months – John found an empty colony, with only the word ‘Croatoan’ carved in a tree as any indication of the settlers’ fate. It remains one of the most intriguing unsolved mysteries of America to this day.
In ‘Croatoan’, Sam and Dean arrived in Rivergrove to investigate a premonition Sam had, but they ended up trapped inside the city.